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Isolated rabbit hearts were perfused within 30 min under conditions of adequate oxygenation in presence and absence (control perfusion) of monoiodacetate prior to total ischemia. Content of nonesterified fatty acids (NEFA) in rabbit hearts was determined under conditions of adequate oxygenation and total ischemia. The total NEFA content in control myocardium was 174 +/- 25 nanomoles per gram of wet weight. Ischemia within 30 min significantly enhanced concentration of NEFA, especially of stearic and arachidonic acids. Prolongation up to 120 min of ischemia caused nonspecific progressive increase in content of NEFA in myocardium. Monoiodacetate increased the concentration of NEFA both in ischemic and in oxygenated myocardium.
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