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Antigens were detected in the blood of rabbits infected with Aspergillus fumigatus by a solid-phase (tube) radioimmunoassay (RIA). The radiolabel for the assay was a polysaccharide-rich alkali extract (APAE) from the mycelia of A. fumigatus. Before this extract could be suitable labeled with 125I, it had to be conjugated with tyramine. Rabbits immunized with heat-killed mycelia had titers of antibody in serum of as high as 1:38,000 against this radiolabeled antigen. With unlabeled and unconjugated APAE as the standard antigen, the sensitivity of the RIA was 12 ng per test, or 500 ng/ml. Antigenemia was detectable by RIA three days after infection of rabbits with A. fumigatus. Blood cultures taken concomitantly were uniformly negative. These results indicate that antigenemia occurs in invasive aspergillus infection and in such cases can be detected by RIA. These observations may be important in the diagnosis of invasive aspergillus infections in humans.
P J Shaffer, and
G Medoff, and
G S Kobayashi
January 1987,
The Journal of infectious diseases,
P J Shaffer, and
G Medoff, and
G S Kobayashi
January 1966,
Acta veterinaria Academiae Scientiarum Hungaricae,
P J Shaffer, and
G Medoff, and
G S Kobayashi
January 1982,
Archiv fur experimentelle Veterinarmedizin,
P J Shaffer, and
G Medoff, and
G S Kobayashi
June 1980,
Annals of internal medicine,
P J Shaffer, and
G Medoff, and
G S Kobayashi
January 1966,
National Institute of Animal Health quarterly,
P J Shaffer, and
G Medoff, and
G S Kobayashi
February 1997,
Experimental parasitology,
P J Shaffer, and
G Medoff, and
G S Kobayashi
January 2007,
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria,
P J Shaffer, and
G Medoff, and
G S Kobayashi
May 1934,
The American journal of pathology,
P J Shaffer, and
G Medoff, and
G S Kobayashi
July 1990,
Microbiologica,
P J Shaffer, and
G Medoff, and
G S Kobayashi
June 1976,
Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique,
