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Antibody Selection via Phage Display in Microtiter Plates. 2023
Stephan Steinke, and
Kristian Daniel Ralph Roth, and
Maximilian Ruschig, and
Nora Langreder, and
Saskia Polten, and
Kai-Thomas Schneider, and
Rico Ballmann, and
Giulio Russo, and
Kilian Johannes Karl Zilkens, and
Maren Schubert, and
Federico Bertoglio, and
Michael Hust
Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.
The most common and robust in vitro technology to generate monoclonal human antibodies is phage display. This technology is a widely used and powerful key technology for recombinant antibody selection. Phage display-derived antibodies are used as research tools, in diagnostic assays, and by 2022, 14 phage display-derived therapeutic antibodies were approved. In this review, we describe a fast high-throughput antibody (scFv) selection procedure in 96-well microtiter plates. The given detailed protocol allows the antibody selection ("panning"), screening, and identification of monoclonal antibodies in less than 2 weeks. Furthermore, we describe an on-rate panning approach for the selection of monoclonal antibodies with fast on-rates.
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