BIOMAT Database Logo BIOMAT Database Logo

Melanoma cell adhesion to defined extracellular matrix components. 1986

P A Netland, and B R Zetter

The binding of mouse B16-F10 melanoma cells to defined extracellular matrix components was studied using a dot-blot cell attachment assay. Radiolabeled melanoma cells adhered to immobilized elastin and laminin. Reduced binding was seen with fibronectin, collagen types I and IV, heparan sulfate, and bovine serum albumin. No adhesive activity was found in elastin associated microfibrillar proteins or in elastin fragments generated by alkali or acid digestion. Adherence to extracellular matrix components has been suggested to play a role in tumor spread to secondary sites. Our results indicate that elastin and laminin are the major extracellular molecules responsible for melanoma cell adhesion.

UI MeSH Term Description Entries
D007797 Laminin Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion. Merosin,Glycoprotein GP-2,Laminin M,Laminin M Chain,Chain, Laminin M,Glycoprotein GP 2,M Chain, Laminin
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008545 Melanoma A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445) Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004549 Elastin alpha-Elastin,kappa-Elastin,alpha Elastin,kappa Elastin
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005353 Fibronectins Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins. Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino

Related Publications

P A Netland, and B R Zetter
Intracellular regulation of cell adhesion to extracellular matrix components in murine B16 melanoma cells.
December 1992, Melanoma research,
P A Netland, and B R Zetter
Echistatin inhibits the adhesion of murine melanoma cells to extracellular matrix components.
January 1995, Biochemistry and molecular biology international,
P A Netland, and B R Zetter
Activated lansoprazole inhibits cancer cell adhesion to extracellular matrix components.
July 1999, International journal of oncology,
P A Netland, and B R Zetter
Exogenous nitric oxide inhibits mesangial cell adhesion to extracellular matrix components.
March 1998, Kidney international,
P A Netland, and B R Zetter
Adhesion of cardiac myocytes to extracellular matrix components.
January 1985, Basic research in cardiology,
P A Netland, and B R Zetter
Episialin (MUC1) overexpression inhibits integrin-mediated cell adhesion to extracellular matrix components.
April 1995, The Journal of cell biology,
P A Netland, and B R Zetter
Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy.
July 2010, Nature protocols,
P A Netland, and B R Zetter
Inhibition by uraemic sera of CD4+ T-cell adhesion to extracellular matrix components.
November 1995, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
P A Netland, and B R Zetter
Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling.
October 2023, Proceedings of the National Academy of Sciences of the United States of America,
P A Netland, and B R Zetter
Growth factors, extracellular matrix components and cell adhesion molecules Warthin's tumor.
May 2001, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
Copied contents to your clipboard!