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Protein kinase C activity has been identified in cytosolic and membrane fractions from rat and rabbit small intestine epithelial cells. The cytosolic fraction comprised about the 75% of total activity. Protein kinase C activity was resolved from other protein kinase activities by ion exchange chromatography. Phosphatidylserine or phosphatidylinositol were required for protein kinase C to be active. In addition, the activity was enhanced by the presence of a diacylglycerol. Diolein and dimyristin were the most effective (13-14 fold activation). In the presence of phosphatidylserine and diolein, the Ka for activation by Ca2+ was 10(-7)M. The phorbol ester TPA substituted for diacylglycerol in activating protein kinase C. Brush border and basolateral membranes contained protein kinase C activity, although the specific activity of the basal lateral membranes was four-fold higher than the specific activity of the brush border membranes. The presence of PKC in small intestine epithelial cells might have important implications in the Ca2+ mediated control of ionic transport in this tissue.
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
February 1982,
The American journal of physiology,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
December 1969,
Fiziologicheskii zhurnal SSSR imeni I. M. Sechenova,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
November 1971,
The Journal of cell biology,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
November 1983,
The Biochemical journal,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
August 1991,
Biochimica et biophysica acta,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
April 1993,
Current eye research,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
March 1998,
Biochimica et biophysica acta,
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
February 1973,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
January 1994,
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994),
G Velasco, and
C F Iglesias, and
P Domínguez, and
F Barros, and
S Gascón, and
P S Lazo
September 1988,
Pflugers Archiv : European journal of physiology,
