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In the present study we have investigated the transfer of phospholipids between vesicles and rat liver mitochondria. Transfer was measured by electron paramagnetic resonance spectroscopy using vesicles that contained spin-labeled phospholipids. A spontaneous transfer was observed which could be strongly inhibited by treating the mitochondria with the thiol reagent mersalyl. Transfer was also greatly reduced after a saline wash of the mitochondria; the transfer activity was then recovered in the wash. This activity was inhibited by tryptic digestion and mersalyl. By gel chromatography, enzyme immunoassay and immunoblotting it was demonstrated that the activity in the wash was due to the nonspecific lipid transfer protein (sterol carrier protein 2). We could estimate that up to 85% of the spontaneous phospholipid transfer between vesicles and rat liver mitochondria was mediated by this transfer protein.
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
October 1988,
Journal of biochemistry,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
March 1994,
European journal of biochemistry,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
October 2004,
Biochemistry,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
February 1989,
Biochimica et biophysica acta,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
May 1995,
Chemistry and physics of lipids,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
May 1990,
Biochemistry,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
February 1985,
Biochemical and biophysical research communications,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
December 1989,
Journal of biochemistry,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
October 1990,
Cell structure and function,
F M Megli, and
A De Lisi, and
A van Amerongen, and
K W Wirtz, and
E Quagliariello
March 2000,
Chemistry and physics of lipids,
