BIOMAT Database Logo BIOMAT Database Logo

[Distribution of DNA sequences recognized by specific endonucleases]. 1977

S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish

The molecular weight distributions of fragments obtained after endonuclease treatment of DNA were studied. DNA's from pigeon and E. coli and restriction enzymes EcoRI and BamHI were used. The samples of 14C- and 3H-labelled DNA were treated by endonucleases, separated electrophoretically in 1% agatose gels, and radioactivity distributions along gels were measured. From these data weight and number distributions and the average molecular weights of DNA fragments were determined. EcoRI-fragments of phage DNA were used as standards for the molecular weight calibration. The experimental results are compared with the expected data calculated from the DNA GC content. The molecular weight distribution of fragments and the average molecular weights of BamHI-fragments of pigeon DNA and EcoRI- and BamHI-fragments of E. coli DNA differed from random ones. It is suggested that certain genomes contain regions in which the probability of endonuclease cleavage strongly differs from the average probability of such a cleavage for the entire genome.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010856 Columbidae Family in the order COLUMBIFORMES, comprised of pigeons or doves. They are BIRDS with short legs, stout bodies, small heads, and slender bills. Some sources call the smaller species doves and the larger pigeons, but the names are interchangeable. Columba livia,Doves,Pigeons,Domestic Pigeons,Feral Pigeons,Rock Doves,Rock Pigeons,Domestic Pigeon,Dove,Dove, Rock,Doves, Rock,Feral Pigeon,Pigeon,Pigeon, Domestic,Pigeon, Feral,Pigeon, Rock,Pigeons, Domestic,Pigeons, Feral,Pigeons, Rock,Rock Dove,Rock Pigeon
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D003201 Computers Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations. Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli

Related Publications

S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis.
January 1980, The Biochemical journal,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Restriction endonucleases or the site-specific DNA endonucleases.
January 1980, Arzneimittel-Forschung,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Novel site-specific DNA endonucleases.
February 1998, Current opinion in structural biology,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Sequence-specific DNA nicking endonucleases.
August 2015, Biomolecular concepts,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
DNA methyltransferases and DNA site-specific endonucleases encoded by chlorella viruses.
January 1993, EXS,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Distribution of globin-specific DNA sequences in fractionated chromatin.
January 1978, The International journal of biochemistry,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe.
January 1991, Molecular and biochemical parasitology,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Occurrence of DNA sequences specifically recognized by drugs in human promoters.
February 2002, Journal of biomolecular structure & dynamics,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site.
September 1978, Gene,
S V Mashko, and Iu I Kozlov, and L V Gening, and A Ia Strongin, and B A Rebentish
Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs.
June 1986, Nucleic acids research,
Copied contents to your clipboard!