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We developed a rapid in vitro test for determining the association of Campylobacter jejuni and C. coli with HeLa cells. Association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (AI). The reproducibility of the AI was checked by repeating the test six times, using four strains chosen at random. Means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. The experimental conditions for which the results are reliable have been standardized. Among 42 strains from human feces, two groups appeared: for 22 nonassociative strains (52%), AI values ranged from 0.0 to 2.1 (mean +/- SD, 0.5 +/- 0.6); for 20 associative strains (48%), AI values ranged from 3.5 to 8.3 (mean +/- SD, 6.2 +/- 1.4). Of these 42 strains, 17 were clinically documented. Diarrhea occurred more frequently in patients infected with associative strains than in those infected with noninvasive strains (7/7 versus 3/10, P = 0.01). Fever also occurred more frequently in patients infected with associative strains (6/7 versus 2/10, P = 0.03). Transmission electron microscopy and viable counts made after killing of extracellular bacteria by gentamicin support the fact that associated Campylobacter spp. are adherent to the cell membrane and are internalized into cytoplasmic vacuoles. The described test seems to be a convenient and rapid method for estimating the pathogenicity of a given strain.
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
January 2009,
Vojnosanitetski pregled,
J L Fauchere, and
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E N Moyen, and
S Richard, and
A Pfister
January 2008,
Revista Argentina de microbiologia,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
March 2005,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
February 1985,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
December 1983,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
August 1988,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
December 1987,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
November 1991,
The Journal of infection,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
November 1984,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
J L Fauchere, and
A Rosenau, and
M Veron, and
E N Moyen, and
S Richard, and
A Pfister
January 1982,
Annali Sclavo; rivista di microbiologia e di immunologia,
