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A new electrophoretic procedure is developed for estimation of glycogen phosphorylase isoforms using acetate cellulose films. In the course of incubation of a mixture containing substrate the reaction was directed towards formation of glucose-I-phosphate, concentration of which was estimated in the coupled reactions based on equimolar increase in content of NADH. Content of the reduced nucleotide was evaluated by means of fluorescent densitometry. Three fractions of glycogen phosphorylase I, II and III were detected after electrophoretic separation of an extract from heart muscle homogenate. Only the isoenzyme I was found in blood serum of patients with myocardium infarction. In subsequent studies clinical validity of the procedure should be evaluated in order to prove the suitability of glycogen phosphorylase isoenzymes estimations for diagnostics of myocardial infarction.
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
January 1989,
Laboratornoe delo,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
January 1973,
Humangenetik,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
March 1972,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
July 1971,
Rinsho byori. The Japanese journal of clinical pathology,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
January 1974,
Acta clinica Belgica,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
June 1967,
Clinica chimica acta; international journal of clinical chemistry,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
July 1972,
Clinical chemistry,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
March 1970,
Clinica chimica acta; international journal of clinical chemistry,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
March 1973,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
I K Filippov, and
V N Titov, and
V I Sargina, and
A Zh Azhmukhanbetova
March 1965,
American journal of clinical pathology,
