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The development of mouse and human embryos was assessed in human amniotic fluid to determine its suitability as a culture medium for human in vitro fertilization (IVF). Two-cell mouse embryos developed to blastocysts after 72 hours at rates similar to that in Whittingham's T6 + 10% fetal calf serum. Significantly more mouse embryos hatched in amniotic fluid. No difference was found between individual patient's amniotic fluids obtained at 16 to 21 weeks' gestation. A preliminary trial comparing amniotic fluid with T6 + maternal serum in human IVF showed no significant difference in fertilization rate and embryo development during 42 to 48 hours in vitro. Expanded blastocysts were obtained in amniotic fluid after 5 days in vitro. Four pregnancies were obtained in 9 patients' transferred embryos grown in amniotic fluid and with 2 or 12 patients' transferred embryos grown in T6 + maternal serum.
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
December 1994,
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L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
January 1998,
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L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
January 1985,
Acta obstetricia et gynecologica Scandinavica. Supplement,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
September 1989,
Fertility and sterility,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
July 1989,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
February 1995,
Ceska gynekologie,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
April 1982,
Harefuah,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
June 1982,
Canadian family physician Medecin de famille canadien,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
July 1985,
Nihon Sanka Fujinka Gakkai zasshi,
L Gianaroli, and
R Seracchioli, and
A P Ferraretti, and
A Trounson, and
C Flamigni, and
L Bovicelli
January 2019,
Methods in molecular biology (Clifton, N.J.),
