Biomaterial Database

Disruption of influenza virus packaging signals results in various misassembled genome complexes. 2023

Justine Girard, and Celia Jakob, and Lina Kathrin Toews, and Jonas Fuchs, and Anne Pohlmann, and Kati Franzke, and Larissa Kolesnikova, and Csaba Jeney, and Martin Beer, and Patrick Bron, and Martin Schwemmle, and Hardin Bolte

Centre de Biologie Structurale, Université de Montpellier, CNRS, INSERM , Montpellier, France.

The influenza A virus genome consists of eight distinct viral RNAs (vRNAs) that are typically packaged into a single virion as an octameric complex. How this genome complex is assembled and incorporated into the virion is poorly understood, but previous research suggests a coordinative role for packaging signals present in all vRNAs. Here, we show that disruption of two packaging signals in a model H7N7 influenza A virus results in a mixture of virions with unusual vRNA content, including empty virions, virions with one to four vRNAs, and virions with octameric complexes composed of vRNA duplicates. Our results suggest that (i) the assembly of error-free octameric complexes proceeds through a series of defined vRNA sub-complexes and (ii) virions can bud without incorporating complete octameric complexes.

UI	MeSH Term	Description	Entries
D009980	Influenza A virus	The type species of the genus ALPHAINFLUENZAVIRUS that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.	Alphainfluenzavirus influenzae,Avian Orthomyxovirus Type A,FLUAV,Fowl Plague Virus,Human Influenza A Virus,Influenza Virus Type A,Influenza Viruses Type A,Myxovirus influenzae-A hominis,Myxovirus influenzae-A suis,Myxovirus pestis galli,Orthomyxovirus Type A,Orthomyxovirus Type A, Avian,Orthomyxovirus Type A, Human,Orthomyxovirus Type A, Porcine,Pestis galli Myxovirus,Fowl Plague Viruses,Influenza A viruses,Myxovirus influenzae A hominis,Myxovirus influenzae A suis,Myxovirus, Pestis galli,Myxoviruses, Pestis galli,Pestis galli Myxoviruses,Plague Virus, Fowl,Virus, Fowl Plague
D000086482	Viral Genome Packaging	Folding of the viral genomic nucleic acid into the VIRUS PARTICLE.	Packaging, Viral,Viral Packaging,Virus Genome Packaging,Packaging, Virus,Genome Packaging, Viral,Genome Packaging, Virus,Genome Packagings, Virus,Packaging, Virus Genome,Packagings, Virus,Packagings, Virus Genome,Virus Genome Packagings,Virus Packaging
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D014771	Virion	The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.	Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D016679	Genome, Viral	The complete genetic complement contained in a DNA or RNA molecule in a virus.	Viral Genome,Genomes, Viral,Viral Genomes
D053126	Influenza A Virus, H7N7 Subtype	A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 7 and neuraminidase 7. The H7N7 subtype produced an epidemic in 2003 which was highly pathogenic among domestic birds (POULTRY). Some infections in humans were reported.	H7N7 Virus,H7N7 subtype,H7N7 Viruses,H7N7 subtypes,Virus, H7N7,subtype, H7N7
D019065	Virus Assembly	The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.	Viral Assembly,Assembly, Viral,Assembly, Virus