-transparent.png){kind=logo}
Adult rat liver slices were maintained for 20 h in a novel organ culture system with minimal loss of viability and function. Potassium and adenosine triphosphate levels were maintained at in vivo levels, following an initial recovery period (2 to 4 h), for up to 20 h. Protein synthesis and secretion were linear for 20 and 16 h, respectively. In addition, the liver slices synthesized glycogen between 4 and 12 h in culture. Finally, the liver slices were hormonally responsive during the 20 h culture period as exemplified by glucagon-stimulated glucose production. This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function.
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
November 1983,
In vitro,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
April 1985,
Life sciences,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
June 1987,
The Anatomical record,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
March 1996,
Drug metabolism and disposition: the biological fate of chemicals,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
September 1971,
Experimental cell research,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
March 1991,
Xenobiotica; the fate of foreign compounds in biological systems,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
January 1988,
Proceedings of the Western Pharmacology Society,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
January 1991,
Drug metabolism and disposition: the biological fate of chemicals,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
January 2007,
Tissue engineering,
P F Smith, and
G Krack, and
R L McKee, and
D G Johnson, and
A J Gandolfi, and
V J Hruby, and
C L Krumdieck, and
K Brendel
December 1976,
Journal of anatomy,
