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Three multipotent mouse teratocarcinoma stem lines, all exhibiting unstable expression for the purine salvage enzyme adenine phosphoribosyltransferase (APRT) were used for the isolation of differentiated cell lines from neoplasms developed in syngeneic mice. Two of the stem cell lines (DAP1B and DAP1C) exhibited homozygous deficiencies for APRT expression while the third stem cell line (E140) exhibited a heterozygous deficiency (Turker, M.S., Smith, A.C., and Martin, G.M.; Somat. Cell Mol. Genet.; 10:55-69; 1984). A total of 16 morphologically differentiated cell lines were established from these neoplasms; most were no longer tumorigenic. Differentiated cell lines derived from the E140-induced tumors segregated homozygous deficient mutants in a single step, consistent with their retention of the heterozygous deficient state. Differentiated homozygous deficient cell lines gave rise to phenotypic revertants at very high frequencies (10(-1) to 10(-2)). The majority of these putative revertants, however, yielded cell-free extracts with little or no detectable APRT activity. These putative revertants were capable of adenine salvage and were therefore termed APRT pseudorevertants. Since the APRT pseudorevertant phenotype was only observed in the differentiated progeny of the APRT deficient stem cell lines, we conclude that this change in the nature of the revertant phenotype was a consequence of cellular differentiation.
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
January 1984,
Somatic cell and molecular genetics,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
November 1979,
Somatic cell genetics,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
December 1985,
Experimental parasitology,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
January 1983,
Gene,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
January 1982,
Differentiation; research in biological diversity,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
July 1990,
Somatic cell and molecular genetics,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
June 1982,
Proceedings of the National Academy of Sciences of the United States of America,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
February 1993,
Cancer letters,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
March 1982,
Molecular and cellular biology,
M S Turker, and
J A Tischfield, and
P Rabinovitch, and
P J Stambrook, and
J J Trill, and
A C Smith, and
C E Ogburn, and
G M Martin
March 1998,
Journal of cellular biochemistry,
