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Using antibody and plaque hybridization screening, we isolated rat argininosuccinate lyase (AS lyase) cDNA clones from a liver cDNA library prepared in the phage expression vector lambda gt11. Five overlapping cDNAs covering 1.7 kilobases of the estimated 2.0-kilobase AS lyase mRNA were characterized and confirmed as AS lyase sequences by hybrid selection. We examined the differential expression of AS lyase in rat liver and four rat hepatoma cell lines (7800C1, H4, HTC, and MH1C1). These cells exhibited a 60-fold range of AS lyase enzyme activity, with a direct correlation between activity, amount of AS lyase immunoreactive protein, and quantity of specific AS lyase mRNA. These observations suggest that the differences in AS lyase expression between rat liver and the hepatoma cell lines result from variations in AS lyase transcriptional activity or alterations in nuclear processing of AS lyase RNA.
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
September 1986,
Biochemistry international,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
October 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
January 1986,
Journal of inherited metabolic disease,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
January 1988,
Journal of biochemistry,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
November 1998,
Gene,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
June 2000,
Biochimica et biophysica acta,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
March 1995,
FEBS letters,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
February 1999,
Molecular & general genetics : MGG,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
May 1995,
Journal of biochemistry,
M A Lambert, and
L R Simard, and
P N Ray, and
R R McInnes
August 1987,
Proceedings of the National Academy of Sciences of the United States of America,
