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The cellular and subcellular localization of one of the gut glucagon-like immunoreactants (GLI-1 or glicentin) and the relative distribution of glicentin- and glucagon-containing cells were investigated by immunocytochemistry. By immunofluorescence, the antiglicentin serum, which does not react with glucagon, revealed positive cells in the islets of Langerhans and in the gut mucosa, particularly in the terminal ileum and colon. In the intestinal mucosa, it was proven ultrastructurally that the glicentin immunoreactive cells correspond to the L cell and that the secretory granules represent the storage compartment of the immunoreactive material. In pancreatic islets, consecutive semithin sections treated with antiglicentin and specific antiglucagon sera showed that the same A cell population reacted with both sera, while immunoperoxidase staining on thin sections revealed that the immunoreactive material was confined to the secretory granules. The same results were obtained on dog oxyntic mucosa, where the glicentin- and glucagon-containing cells were identified as the gastric A cell. The immunocytochemical demonstration of a common glicentin-like material in the A and L cells together with the known presence of a common immunoreactant in glicentin and glucagon strongly support the idea that the A and L cells are ontogenetically related and synthesize their secretory product via a glicentin-like precursor which, by specific cleavage, could yield glucagon and gut glucagon-like immunoreactants.
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
January 1982,
Cell and tissue research,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
January 1980,
Cell and tissue research,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
February 1981,
Nature,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
February 1980,
Diabetes,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
January 1987,
Histochemistry,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
June 1982,
Endocrinology,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
May 1978,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
June 1988,
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
June 1983,
Gastroenterology,
M Ravazzola, and
A Siperstein, and
A J Moody, and
F Sundby, and
H Jacobsen, and
L Orci
September 1981,
Nihon rinsho. Japanese journal of clinical medicine,
