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The presence of the proenkephalin A-derived peptide Met5-enkephalin-Arg6-Gly7-Leu8 was demonstrated throughout the human gastrointestinal tract. Highest concentrations of Met5-enkephalin-Arg6-Gly7-Leu8, as assessed by radioimmunoassay, were measured in the separated muscularis externa, while lower levels were found in the submucosa and only small amounts in the mucosa. The results are consistent with a neuronal location of this peptide in the human gut. Over 65% of total immunoreactivity coeluted with the authentic peptide in both molecular exclusion chromatography and HPLC, while most of the remainder activity eluted earlier on gel filtration. The latter material probably represents N-terminally extended Met5-enkephalin-Arg6-Gly7-Leu8. Taken together with previous studies, our results appear to indicate that there are important species differences in post-translational processing of proenkephalin A in gut nerves.
G L Ferri, and
R A Morreale, and
G J Dockray
January 1987,
European journal of pharmacology,
G L Ferri, and
R A Morreale, and
G J Dockray
October 1991,
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G L Ferri, and
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G J Dockray
March 1987,
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G L Ferri, and
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G J Dockray
June 1989,
Neuroscience letters,
G L Ferri, and
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G L Ferri, and
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G L Ferri, and
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April 1986,
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G L Ferri, and
R A Morreale, and
G J Dockray
September 1987,
Clinical and experimental pharmacology & physiology,
G L Ferri, and
R A Morreale, and
G J Dockray
April 1987,
Neuroscience,
G L Ferri, and
R A Morreale, and
G J Dockray
March 1985,
Brain research,
