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In order to develop a radioimmunoassay for the new progestagen dienogest (STS 557, 17 alpha-cyanomethyl-17 beta-hydroxy-estra-4,9-dien-3-one), bovine serum albumin (BSA) conjugates of STS 557-3-carboxymethyloxime and of STS 557-11-hemisuccinate were synthesized as antigens for the production of antisera. It was proved that an excess of isobutylchlorocarbonate in the coupling reaction using the "mixed anhydride method" results in an acylation of free NH2-groups in the BSA. By the immunization of rabbits with the STS 557-antigen-antisera of high specificity and affinity to STS 557 were produced. Endogenous steroids show no cross reaction with the STS 557-antisera. Steroids with a 17 alpha-CH2CN-group, being obtained by chemical synthesis or microbial transformation, compete with STS 557 for the binding positions of the antibodies to a different extent.
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
February 2003,
Journal of agricultural and food chemistry,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
May 1979,
Journal of steroid biochemistry,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
March 2018,
Molecules (Basel, Switzerland),
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
January 2004,
Bioorganicheskaia khimiia,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
April 1989,
The Journal of pharmacy and pharmacology,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
September 2006,
Bioscience, biotechnology, and biochemistry,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
May 1995,
Biochemical Society transactions,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
October 2020,
Food chemistry,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
April 2022,
The journal of physical chemistry. B,
G Hobe, and
Do Khac Hieu, and
G Reddersen, and
R Wesemann, and
M Hübner, and
B Menzenbach, and
H Wagner, and
J Römer, and
G Frankenberg, and
K Schubert
January 2005,
Bioconjugate chemistry,
