-transparent.png){kind=logo}
We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60 degrees C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
September 1976,
Biochimica et biophysica acta,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
January 1977,
Biochimica et biophysica acta,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
January 1982,
Arteriosclerosis (Dallas, Tex.),
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
January 1985,
Journal of lipid research,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
May 1987,
Analytical biochemistry,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
February 2001,
Nihon rinsho. Japanese journal of clinical medicine,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
January 1984,
Agents and actions. Supplements,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
April 1985,
Biochimica et biophysica acta,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
February 1979,
European journal of biochemistry,
W L Crump, and
B C Sherrill, and
A M Gotto, and
G Ghiselli
January 1976,
Journal of lipid research,
