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A protein having Mr of more than 900K (900,000) was isolated by chromatography on a column of Sepharose 4B coupled with solubilized elastin, followed by sucrose density gradient centrifugation. The protein is composed of several disulfide-linked subunits. SDS-polyacrylamide gel electrophoresis and immunoblotting indicated that two of the subunits are identical with the heavy chains of IgM and IgG, and one is immunologically related to the heavy chain of IgA. The results suggested that this protein is a new protein that belongs to the category of, or is closely related to, the immunoglobulins. As described in the succeeding paper, it has cell-binding activity (Fukamizu et al. (1986) J. Biochem. 100, 843-848). We tentatively refer to this protein as "cell-binding immunoglobulin-like protein (CIP)."
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
September 1990,
Biochimica et biophysica acta,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
January 1984,
Preparative biochemistry,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
July 1978,
The Journal of experimental medicine,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
January 1979,
Annals of the New York Academy of Sciences,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
August 1991,
Journal of immunological methods,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
October 1996,
Journal of biochemistry,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
March 1991,
European journal of biochemistry,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
July 1986,
The Journal of biological chemistry,
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
March 1972,
Journal of immunology (Baltimore, Md. : 1950),
K Ohori, and
H Fukamizu, and
K Matsushita, and
K Horiuchi, and
D Fujimoto
September 2002,
Biochemistry. Biokhimiia,
