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A micro enzyme-linked immunoassay (ELISA) is described for the quantitation of secretory IgA as distinct from monomeric IgA. The assay is sensitive (linear down to 30 ng/ml) and reproducible (inter-assay variation: 17.0%; intra-assay variation: 11.5%). The assay has the further advantages of rapidity, the ability to handle large numbers of samples, and uses commercially available reagents throughout. Minimal interference from a large (greater than 25-fold) excess of monomeric IgA was observed. The results obtained for serum secretory IgA concentrations by this method correlated well with those reported by other workers for normal control patients, patients with IgA deficiency and patients with liver disease.
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
October 1987,
Journal of immunological methods,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
September 1981,
Lancet (London, England),
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
February 1998,
Journal of immunology (Baltimore, Md. : 1950),
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
January 1985,
The Journal of experimental medicine,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
December 1977,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
January 1989,
Clinical physiology and biochemistry,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
April 2006,
Nippon Ganka Gakkai zasshi,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
February 1986,
Clinica chimica acta; international journal of clinical chemistry,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
February 1990,
Nihon Jinzo Gakkai shi,
G M Wood, and
L K Trejdosiewicz, and
M S Losowsky
March 1995,
Journal of immunological methods,
