BIOMAT Database Logo BIOMAT Database Logo

Methods for in vitro percutaneous absorption studies. VII: Use of excised human skin. 1986

R L Bronaugh, and R F Stewart, and M Simon

Water permeability constants (Kp) were determined with skin from human cadavers. No difference was seen in Kp values from unfrozen skin or from skin frozen for a few days. Human skin could usually be stored at -20 degrees C for up to a year with no change in water permeability, but in some cases apparent deterioration of the barrier was observed. A rapid procedure was developed for checking barrier integrity of skin in diffusion cells before a penetration study. The percent of the water dose absorbed after 20-min contact with skin correlated with water Kp values. Changes in water permeation through human skin agreed with changes in the absorption of seven test compounds of varying solubility properties (acetylsalicylic acid, benzo(a)pyrene, cortisone, DDT, nicotinic acid, propylene glycol, and testosterone). Water permeation is therefore considered to be a good indicator of potential changes in the barrier integrity of human skin. No correlation was observed in Kp values and other characteristics of the donor skin samples such as age, sex, race, and length of time before skin harvest.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D001834 Body Water Fluids composed mainly of water found within the body. Water, Body
D004058 Diffusion The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT. Diffusions
D004364 Pharmaceutical Preparations Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form. Drug,Drugs,Pharmaceutical,Pharmaceutical Preparation,Pharmaceutical Product,Pharmaceutic Preparations,Pharmaceutical Products,Pharmaceuticals,Preparations, Pharmaceutical,Preparation, Pharmaceutical,Preparations, Pharmaceutic,Product, Pharmaceutical,Products, Pharmaceutical
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012869 Skin Absorption Uptake of substances through the SKIN. Absorption, Skin,Intracutaneous Absorption,Intradermal Absorption,Percutaneous Absorption,Transcutaneous Absorption,Transdermal Absorption,Absorption, Intracutaneous,Absorption, Intradermal,Absorption, Percutaneous,Absorption, Transcutaneous,Absorption, Transdermal,Absorptions, Intracutaneous,Absorptions, Intradermal,Absorptions, Percutaneous,Absorptions, Skin,Absorptions, Transcutaneous,Absorptions, Transdermal,Intracutaneous Absorptions,Intradermal Absorptions,Percutaneous Absorptions,Skin Absorptions,Transcutaneous Absorptions,Transdermal Absorptions
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

R L Bronaugh, and R F Stewart, and M Simon
[Studies on corticosteroid ointments. VI. Percutaneous absorption of hydrocortisone-4-14C. 2. Percutaneous absorption using human excised skin in vitro].
September 1969, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
R L Bronaugh, and R F Stewart, and M Simon
In vitro percutaneous absorption and metabolism of ozenoxacin in excised human skin.
January 2014, Future microbiology,
R L Bronaugh, and R F Stewart, and M Simon
Skin culture model: a possible alternative to the use of excised human skin for assessing in vitro percutaneous absorption.
August 1998, Toxicology in vitro : an international journal published in association with BIBRA,
R L Bronaugh, and R F Stewart, and M Simon
[Studies on corticosteroid ointments. V. Percutaneous absorption of hydrocortisone-4-14C. (1). In vitro method of percutaneous absorption using excised human skin].
March 1969, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
R L Bronaugh, and R F Stewart, and M Simon
Parallel evaluation of alternative skin barrier models and excised human skin for dermal absorption studies in vitro.
September 2023, Toxicology in vitro : an international journal published in association with BIBRA,
R L Bronaugh, and R F Stewart, and M Simon
The influence of liquid crystalline phases on drug percutaneous absorption. II. Permeation studies through excised human skin.
December 1992, Pharmaceutical research,
R L Bronaugh, and R F Stewart, and M Simon
In vitro percutaneous absorption of benzene in human skin.
January 2009, Cutaneous and ocular toxicology,
R L Bronaugh, and R F Stewart, and M Simon
Percutaneous absorption of captopril from hydrophilic cellulose derivatives through excised rabbit skin and human skin.
February 1998, Drug development and industrial pharmacy,
R L Bronaugh, and R F Stewart, and M Simon
Studies on percutaneous penetration of chemicals - Impact of storage conditions for excised human skin.
March 2013, Toxicology in vitro : an international journal published in association with BIBRA,
R L Bronaugh, and R F Stewart, and M Simon
Anchored skin equivalent cultured in vitro: a new tool for percutaneous absorption studies.
November 1993, In vitro cellular & developmental biology. Animal,
Copied contents to your clipboard!