Development and characterization of a hemolysis inhibition assay to determine functionality of anti-Streptolysin O antibodies in human sera. 2024

Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
GSK Vaccines Institute for Global Health (GVGH), GSK Global Health Vaccines R&D, via Fiorentina 1, 53100 Siena, Italy.

The high burden of disease and the long-lasting sequelae following Streptococcus pyogenes (Strep A) infections make the development of an effective vaccine a global health priority. Streptolysin O (SLO), is a key toxin in the complex pathogenesis of Strep A infection. Antibodies are elicited against SLO after natural exposure and represent a key target for vaccine-induced immunity. Here we present the setup and characterization of a hemolysis assay to measure functionality of anti-SLO antibodies in human sera. Assay specificity, precision, linearity, reproducibility, and repeatability were determined. The assay was demonstrated to be highly sensitive, specific, reproducible, linear and performed well in assessing functionality of anti-SLO antibodies induced by exposed individuals. Moreover, different sources of critical reagents, in particular red- blood cells, have been compared and had minimal impact on assay performance. The assay presented here has throughput suitable for evaluating sera in vaccine clinical trials and sero-epidemiological studies to gain further insights into the functionality of infection- and vaccine-induced antibodies.

UI MeSH Term Description Entries
D006461 Hemolysis The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity. Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D013290 Streptococcal Infections Infections with bacteria of the genus STREPTOCOCCUS. Group A Strep Infection,Group A Streptococcal Infection,Group A Streptococcal Infections,Group B Strep Infection,Group B Streptococcal Infection,Group B Streptococcal Infections,Infections, Streptococcal,Infection, Streptococcal,Streptococcal Infection
D013297 Streptococcus pyogenes A species of gram-positive, coccoid bacteria isolated from skin lesions, blood, inflammatory exudates, and the upper respiratory tract of humans. It is a group A hemolytic Streptococcus that can cause SCARLET FEVER and RHEUMATIC FEVER. Flesh-Eating Bacteria,Streptococcus Group A,Bacteria, Flesh-Eating
D013301 Streptolysins Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS. Streptolysin
D014612 Vaccines Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases. Vaccine
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face

Related Publications

Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
January 1957, Rivista dell'Istituto sieroterapico italiano,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
January 1985, Microbiology and immunology,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
November 1961, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
October 1986, Journal of immunology (Baltimore, Md. : 1950),
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
March 1980, Quaderni Sclavo di diagnostica clinica e di laboratorio,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
January 1987, Haematologica,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
March 2024, Journal of immunological methods,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
July 2022, Biotech (Basel (Switzerland)),
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
December 1997, Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne,
Martina Carducci, and Alana Whitcombe, and Luca Rovetini, and Luisa Massai, and Alexander J Keeley, and Thushan I de Silva, and Julie Bennett, and Francesco Berlanda Scorza, and Miren Iturriza, and Nicole J Moreland, and Danilo G Moriel, and Omar Rossi
January 1986, Journal of clinical microbiology,
Copied contents to your clipboard!