-transparent.png){kind=logo}
An indirect enzyme-linked immunosorbent assay (ELISA) was developed which was specific and sensitive in detecting antibodies to Ehrlichia risticii in Potomac horse fever (PHF). The ELISA antibody titers were correlated with the indirect fluorescent antibody (IFA) titers. E. risticii propagated in human histiocyte culture was purified on renografin gradient and the band of the organisms at a density of 1.182 g/ml was used as antigen. ELISA antibody titers were determined through computer assisted analysis, the observed antibody titers were derived by serial serum dilutions and using a resultant standard curve the predicted antibody titers were obtained from a single serum dilution. The standard curve had a correlation coefficient of 0.8975. The observed and predicted antibody titers were in good agreement, as the respective titers fell within a two-fold range. There was a good correlation between ELISA and IFA test results, but the ELISA titers were several times higher. In experimental infections of horses produced with the infected equine whole blood and the Ehrlichia infected macrophage culture, the antibodies were first detected in two weeks and one week postinoculation (PI), respectively. In both cases the titers reached a peak in about 4 weeks PI with a mean titer of 1:16558 and 1:4030, respectively. The antibody titers of the convalescent sera of field cases of PHF were comparatively lower than the experimentally infected horses.
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
January 1987,
Journal of clinical microbiology,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
May 1991,
Veterinary microbiology,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
June 1988,
The Veterinary record,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
January 1993,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
January 1990,
Annals of the New York Academy of Sciences,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
November 1989,
Parasitology today (Personal ed.),
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
January 1984,
Acta veterinaria Scandinavica,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
January 2001,
The Veterinary record,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
June 2002,
Comparative medicine,
S K Dutta, and
R M Rice, and
T D Hughes, and
P K Savage, and
A C Myrup
October 1991,
Journal of clinical microbiology,
