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A simple and rapid method for studying optical anisotropic properties of liposomes was proposed. Intensities of transmitted light through one spherical liposome of dipalmitoylphosphatidylcholine placed between two polarizers were measured at various wavelengths by a microscopic spectrophotometer. Large increases in the intensities were observed at the prephase-transition temperature, which were caused by an increase in the birefringence of the multilayer of the liposome. The birefringence values obtained from the intensity data were about 0.020 below the pretransition temperature and 0.028 above that temperature. These values are in good agreement with the results reported for the plane samples in which lipid bilayers are stacked. The obtained values of the birefringence were far lower than the values estimated from polarizabilities. This lower birefringence is attributed to disordering of the tilt direction in the multilayer. The degree of order of the liposome multilayers calculated from the birefringence increased by 38% at the pretransition. This simple method is applicable to the study of the multilayer structure of liposomes in water.
K Mishima, and
K Satoh, and
T Ogihara
June 1995,
Biophysical journal,
K Mishima, and
K Satoh, and
T Ogihara
March 1993,
Biochimica et biophysica acta,
K Mishima, and
K Satoh, and
T Ogihara
June 1982,
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K Mishima, and
K Satoh, and
T Ogihara
August 1982,
Chemistry and physics of lipids,
K Mishima, and
K Satoh, and
T Ogihara
September 1979,
Biophysical journal,
K Mishima, and
K Satoh, and
T Ogihara
November 1991,
Biochemical Society transactions,
K Mishima, and
K Satoh, and
T Ogihara
August 1989,
Biochemical and biophysical research communications,
K Mishima, and
K Satoh, and
T Ogihara
September 1974,
European journal of biochemistry,
K Mishima, and
K Satoh, and
T Ogihara
February 1984,
Indian journal of biochemistry & biophysics,
K Mishima, and
K Satoh, and
T Ogihara
November 1982,
Biochimica et biophysica acta,
