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Cortisone 5 beta-reductase (4,5 beta-dihydrocortisone:NADP+ delta 4-oxidoreductase, EC 1.3.1.3) was purified from rat liver 100,000 X g supernate to a homogeneous state based on the catalytic activity. In the course of purification the activity was always accompanied by androstenedione 5 beta-reductase (3-oxo-5 beta-steroid:NADP+ delta 4-oxidoreductase, EC 1.3.1.23) and no fraction which revealed only cortisone 5 beta-reductase activity but lacked androstenedione 5 beta-reductase was observed. Partial denaturation of the purified enzyme with p-chloromercuribenzoate or wtih heat reduced both enzyme activities to a similar extent. When both substrates were added together at concentrations sufficient to saturate or nearly saturate the enzyme when added separately, the total rate of the reactions was much less than the sum of the rates of the reactions measured separately. Judging from these results it was concluded that cortisone 5 beta-reduction and that of androstenedione are catalyzed by the same catalytic site of a single protein.
M Furuebisu, and
S Deguchi, and
K Okuda
June 1984,
The Journal of biological chemistry,
M Furuebisu, and
S Deguchi, and
K Okuda
November 1989,
Chemical & pharmaceutical bulletin,
M Furuebisu, and
S Deguchi, and
K Okuda
May 1973,
The Journal of clinical endocrinology and metabolism,
M Furuebisu, and
S Deguchi, and
K Okuda
September 1985,
Biochimica et biophysica acta,
M Furuebisu, and
S Deguchi, and
K Okuda
January 1996,
Hepatology (Baltimore, Md.),
M Furuebisu, and
S Deguchi, and
K Okuda
January 1998,
Ryoikibetsu shokogun shirizu,
M Furuebisu, and
S Deguchi, and
K Okuda
June 1985,
The Journal of biological chemistry,
M Furuebisu, and
S Deguchi, and
K Okuda
June 1991,
FEBS letters,
M Furuebisu, and
S Deguchi, and
K Okuda
March 2001,
Nihon rinsho. Japanese journal of clinical medicine,
M Furuebisu, and
S Deguchi, and
K Okuda
December 1993,
Molecular endocrinology (Baltimore, Md.),
