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Structure of acidic oligosaccharides isolated from pronase-treated glycoprotein of bonnet-monkey (Macaca radiata) cervical mucus. 1987

Nasir-ud-Din

The major glycoprotein component of cervical mucus of bonnet monkey was treated with Pronase, and the enzyme-resistant glycoprotein purified by gel filtration on Sepharose 4B followed by DEAE-cellulose chromatography. Alkaline-borohydride cleavage of the carbohydrate chains gave a mixture of neutral and acidic oligosaccharides. Seven acidic oligosaccharides were characterized by chemical and enzymic procedures; their proposed structures are: alpha NeuAc(2----3)-[beta GalNAc(1----4)]beta Gal(1----4)GlcNAc(1----6)[alpha Fuc(1----2)beta Gal(1----3)]GalNAc-ol; alpha Fuc(1----2)beta Gal(1----3)/6)[alpha NeuAc(2----3)beta Gal(1----4)GlcNAc(1----3)/6)]GalNAc-ol; alpha GalNAc(1----3)beta Gal(1----3)[alpha NeuAc(2----3)beta Gal(1----4)GlcNAc(1----6)]GalNAc-ol; beta GlcNAc(1----3)[alpha Fuc(1----2)]beta Gal(1----3)[alpha NeuAc(2----6)]GalNAc-ol; beta Gal(1----3)-[alpha NeuAc(2----6)]GalNAc-ol; alpha NeuAc(2----6)GalNAc-ol; and beta Gal3SO3(1----4) GlcNAc(1----6)[alpha Fuc(1----2)beta Gal(1----3)]GalNAc-ol.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008255 Macaca radiata A species of macaque monkey that mainly inhabits the forest of southern India. They are also called bonnet macaques or bonnet monkeys. Bonnet Monkey,Monkey, Bonnet,Bonnet Macaques,Bonnet Macaque,Bonnet Monkeys,Macaca radiatas,Macaque, Bonnet,Macaques, Bonnet,Monkeys, Bonnet,radiata, Macaca
D009844 Oligosaccharides Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form. Oligosaccharide
D011402 Pronase A proteolytic enzyme obtained from Streptomyces griseus. Pronase E,Pronase P,Protease XIV,XIV, Protease
D002236 Carbohydrate Conformation The characteristic 3-dimensional shape of a carbohydrate. Carbohydrate Linkage,Carbohydrate Conformations,Carbohydrate Linkages,Conformation, Carbohydrate,Conformations, Carbohydrate,Linkage, Carbohydrate,Linkages, Carbohydrate
D002240 Carbohydrate Sequence The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS. Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D002582 Cervix Mucus A slightly alkaline secretion of the endocervical glands. The consistency and amount are dependent on the physiological hormone changes in the menstrual cycle. It contains the glycoprotein mucin, amino acids, sugar, enzymes, and electrolytes, with a water content up to 90%. The mucus is a useful protection against the ascent of bacteria and sperm into the uterus. (From Dictionary of Obstetrics and Gynecology, 1988) Fern Test,Palm-Leaf Reaction,Cervical Mucus,Fern Tests,Mucus, Cervical,Mucus, Cervix,Palm Leaf Reaction,Palm-Leaf Reactions,Reaction, Palm-Leaf,Reactions, Palm-Leaf,Test, Fern,Tests, Fern
D002848 Chromatography, DEAE-Cellulose A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) DEAE-Cellulose Chromatography,Chromatography, DEAE Cellulose,DEAE Cellulose Chromatography
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D005260 Female Females

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