Biomaterial Database

Presence of nonesterified and acylcarnitine in human polymorphonuclear leukocytes and mononuclear cells. 1987

K Katrib, and H A Adlouni, and G Férard

We demonstrate the presence of nonesterified carnitine and acylcarnitine in leukocytes, but not in erythrocytes, from 16 healthy adults. After carefully separating the different kinds of blood cells we measured significant amounts of nonesterified carnitine and acylcarnitine in polymorphonuclear leukocytes (28.5 +/- 6.1 and 18.5 +/- 6.3 mumol/10(9) cells) and mononuclear cells (25.4 +/- 5.2 and 14.8 +/- 4.5 mumol/10(9) cells). We also measured nonesterified carnitine, long-chain acylcarnitine, and short-chain acylcarnitine in plasma after fractionation with perchloric acid and obtained the following values (mean +/- SD): 41.4 +/- 2.6, 3.9 +/- 1.2, and 6.0 +/- 1.6 mumol/L, respectively. The mean percentages of total carnitine (n = 6) in polymorphonuclear leukocytes, mononuclear cells, and plasma were approximately 62%, 27%, and 13% of whole-blood carnitine, respectively (mean recovery was 102%). The percentage of acylated carnitine was 37% in leukocytes, as compared with 19% in plasma.

UI	MeSH Term	Description	Entries
D009000	Monocytes	Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.	Monocyte
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D002331	Carnitine	A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.	Bicarnesine,L-Carnitine,Levocarnitine,Vitamin BT,L Carnitine
D004734	Energy Metabolism	The chemical reactions involved in the production and utilization of various forms of energy in cells.	Bioenergetics,Energy Expenditure,Bioenergetic,Energy Expenditures,Energy Metabolisms,Expenditure, Energy,Expenditures, Energy,Metabolism, Energy,Metabolisms, Energy
D004952	Esters	Compounds derived from organic or inorganic acids in which at least one hydroxyl group is replaced by an –O-alkyl or another organic group. They can be represented by the structure formula RCOOR’ and are usually formed by the reaction between an acid and an alcohol with elimination of water.	Ester
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000215	Acylation	The addition of an organic acid radical into a molecule.