-transparent.png){kind=logo}
Using a synthetic progestin (P)(i.e. R5020), characteristics of P receptors were determined in the ovarian tissue cytosols from adult estradiol benzoate-treated or 4-day cycling rats. In the estradiol benzoate-treated rats a specific 3H-R5020 binding in the cytosol was found with a number of binding sites, V max = 110 fmol/mg protein and the equilibrium dissociation constant, Kd = 14 nM. In the 4-day cycling rats, specific binding was found in the 6-7 S region with the Vmax which fluctuated during the estrous cycle: the sequence of Vmax (fmol/mg protein) was 395 (proestrus) greater than 122 (diestrus) greater than 96 (late estrus) greater than 62 (metestrus) greater than 40 (early estrus). The Kd value varied during the cycle, the highest (22 nM) in proestrus and the lowest (5 nM) in early estrus. In addition, 3H-R5020 binders were thermolabile and of protein in nature. These results suggest that the rat ovary contains P receptors in its cytoplasm with high affinity and low capacity of P binding, the level of which fluctuates during the estrous cycle.
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
August 1989,
Endocrinology,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
June 1985,
Asia-Oceania journal of obstetrics and gynaecology,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
April 2012,
Systems biology in reproductive medicine,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
January 1980,
Journal of steroid biochemistry,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
May 1988,
Acta endocrinologica,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
January 1998,
Life sciences,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
January 1964,
Acta cytologica,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
January 1996,
European journal of biochemistry,
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
August 1980,
Science (New York, N.Y.),
H Fujii-Hanamoto, and
K Seiki, and
K Sakabe, and
Y Haruki, and
H Ogawa
September 1984,
Biochemical and biophysical research communications,
