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A new assay for pyrimidine nucleoside phosphorylase is reported. This method utilizes an isocratic reversed-phase high-performance liquid chromatographic system for separation of nucleosides and bases. Product detection is accompanied by ultraviolet monitoring and radioactive flow detection. Use of an automated sample injector allows for the analysis of a series of samples, with data recorded onto a microprocessor-based cassette recorder. Data can then be downloaded into computer memory. The velocity of uridine phosphorylase (E.C. 2.4.2.3) was a linear function of enzyme concentration. The Michaelis constant for uridine at pH 8.0 was found to be in close agreement with the value obtained by a thin-layer chromatographic assay method.
S A Siegel, and
K L Woods, and
R N Dreyer
November 1988,
Clinica chimica acta; international journal of clinical chemistry,
S A Siegel, and
K L Woods, and
R N Dreyer
October 1980,
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S A Siegel, and
K L Woods, and
R N Dreyer
May 1983,
Chemical & pharmaceutical bulletin,
S A Siegel, and
K L Woods, and
R N Dreyer
May 1995,
Analytical biochemistry,
S A Siegel, and
K L Woods, and
R N Dreyer
June 2010,
Nucleosides, nucleotides & nucleic acids,
S A Siegel, and
K L Woods, and
R N Dreyer
May 1984,
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S A Siegel, and
K L Woods, and
R N Dreyer
October 1990,
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S A Siegel, and
K L Woods, and
R N Dreyer
December 1977,
Clinical chemistry,
S A Siegel, and
K L Woods, and
R N Dreyer
October 1995,
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S A Siegel, and
K L Woods, and
R N Dreyer
June 2005,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
