-transparent.png){kind=logo}
A small ColE1 derivative, pAO2, which replicates like the original ColE1 and confers immunity to colicin E1 on its host cell has been constructed from a quarter region of ColE1 DNA (Oka, 1978). The entire nucleotide sequence of pAO2 (1,613 base pairs) was determined based on its fine cleavage map. The sequence of a similar plasmid, pAO3, carrying additional 70 base pairs was also deduced. The sequence in the region covering the replication initiation site on these plasmids was consistent with those reported for ColE1 by Tomizawa et al. (1977) and by Bastia (1977). DNA sequences indispensable for autonomous replication were examined by constructing plasmids from various restriction fragments of pAO2 DNA. As a result, a region of 436 base pairs was found to contain sufficient information to permit replication. The occurrence of initiation and termination codons and of the ribosome-binding sequence on pAO2 DNA suggests that a polypeptide chain consisting of 113 amino acid residues may be encoded by the region in which the colicin E1 immunity gene has been mapped.
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
July 1982,
Journal of biochemistry,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
February 1978,
Journal of bacteriology,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
September 1978,
Molecular & general genetics : MGG,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
January 1978,
Current topics in microbiology and immunology,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
June 1986,
Genetika,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
February 1978,
Gene,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
October 1979,
Molecular & general genetics : MGG,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
November 1981,
Gene,
A Oka, and
N Nomura, and
M Morita, and
H Sugisaki, and
K Sugimoto, and
M Takanami
February 1984,
Journal of biochemistry,
