Temporal transcriptomic dynamics in developing macaque neocortex. 2024

Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
Institute of Medical Biology Chinese Academy of Medical Sciences, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China.

Despite intense research on mice, the transcriptional regulation of neocortical neurogenesis remains limited in humans and non-human primates. Cortical development in rhesus macaque is known to recapitulate multiple facets of cortical development in humans, including the complex composition of neural stem cells and the thicker supragranular layer. To characterize temporal shifts in transcriptomic programming responsible for differentiation from stem cells to neurons, we sampled parietal lobes of rhesus macaque at E40, E50, E70, E80, and E90, spanning the full period of prenatal neurogenesis. Single-cell RNA sequencing produced a transcriptomic atlas of developing parietal lobe in rhesus macaque neocortex. Identification of distinct cell types and neural stem cells emerging in different developmental stages revealed a terminally bifurcating trajectory from stem cells to neurons. Notably, deep-layer neurons appear in the early stages of neurogenesis, while upper-layer neurons appear later. While these different lineages show overlap in their differentiation program, cell fates are determined post-mitotically. Trajectories analysis from ventricular radial glia (vRGs) to outer radial glia (oRGs) revealed dynamic gene expression profiles and identified differential activation of BMP, FGF, and WNT signaling pathways between vRGs and oRGs. These results provide a comprehensive overview of the temporal patterns of gene expression leading to different fates of radial glial progenitors during neocortex layer formation.

UI MeSH Term Description Entries
D008253 Macaca mulatta A species of the genus MACACA inhabiting India, China, and other parts of Asia. The species is used extensively in biomedical research and adapts very well to living with humans. Chinese Rhesus Macaques,Macaca mulatta lasiota,Monkey, Rhesus,Rhesus Monkey,Rhesus Macaque,Chinese Rhesus Macaque,Macaca mulatta lasiotas,Macaque, Rhesus,Rhesus Macaque, Chinese,Rhesus Macaques,Rhesus Macaques, Chinese,Rhesus Monkeys
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D058953 Neural Stem Cells Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA. Cell, Neural Stem,Cells, Neural Stem,Neural Stem Cell,Stem Cell, Neural,Stem Cells, Neural
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile
D019579 Neocortex The largest portion of the CEREBRAL CORTEX in which the NEURONS are arranged in six layers in the mammalian brain: molecular, external granular, external pyramidal, internal granular, internal pyramidal and multiform layers. Cerebral Neocortex,External Granular Layer,Isocortex,Neocortical External Granular Layer,Neocortical External Pyramidal Layer,Neocortical Internal Granular Layer,Neocortical Internal Pyramidal Layer,Neocortical Molecular Layer,Neocortical Multiform Layer,Neopallial Cortex,Neopallium,Substantia Corticalis,Cerebral Neocortices,Cortex, Neopallial,Corticali, Substantia,Corticalis, Substantia,Cortices, Neopallial,External Granular Layers,Granular Layer, External,Granular Layers, External,Isocortices,Layer, External Granular,Layer, Neocortical Molecular,Layer, Neocortical Multiform,Layers, External Granular,Layers, Neocortical Molecular,Layers, Neocortical Multiform,Molecular Layer, Neocortical,Molecular Layers, Neocortical,Multiform Layer, Neocortical,Multiform Layers, Neocortical,Neocortex, Cerebral,Neocortical Molecular Layers,Neocortical Multiform Layers,Neocortices,Neocortices, Cerebral,Neopallial Cortices,Neopalliums,Substantia Corticali
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays

Related Publications

Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
January 2020, Cerebral cortex communications,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
September 2019, Nature,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
August 2016, Journal of neurophysiology,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
October 1998, Nature,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
November 2004, Journal of neurophysiology,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
April 2020, Nature,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
April 2005, Journal of neurophysiology,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
January 2002, Results and problems in cell differentiation,
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
May 2019, Science (New York, N.Y.),
Longjiang Xu, and Zan Yuan, and Jiafeng Zhou, and Yuan Zhao, and Wei Liu, and Shuaiyao Lu, and Zhanlong He, and Boqin Qiang, and Pengcheng Shu, and Yang Chen, and Xiaozhong Peng
March 2010, Journal of neurophysiology,
Copied contents to your clipboard!