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A phosphamide nucleotide analog: a substrate for polymerase synthesis of DNA. 2024
Jiong Meng, and
Qiaqia Guo, and
Xiaona Zhai, and
Song Yang, and
Shuai Wang, and
Pengcheng Wang, and
Debin Ji
Institute of Frontier Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Qingdao, 266237, PR China. debinji@sdu.edu.cn.
A type of modified nucleotide, deoxynucleotide γ-amidotriphosphates (dNTPγNH2s), exhibited around five times higher stability than dNTPs. These phosphamide nucleotides can be utilized by several DNA polymerases, and the amplification of a 10 kb DNA fragment through the polymerase chain reaction (PCR) can be accomplished even under conditions of high temperature, extended storage, or repeated freeze-thaw cycles. However, the control PCR with standard dNTPs was unsuccessful. These results indicate that dNTPγNH2s have the potential to substitute dNTPs in PCR.
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