Biomaterial Database

Expression of YBB 3/10 antigen in human tumor colon cell lines and its induction by N,N-dimethylformamide. 1985

A Quaroni

A monoclonal antibody (YBB 3/10) raised against rat small intestinal brush border membranes defined a high-molecular-weight antigen present on the surface membrane of the epithelial cells in fetal and newborn rat small intestine but appeared specific for the undifferentiated crypt cells in adult rat small intestine. In rat colon and rectum, expression of the YBB 3/10 antigen was limited to a specific period of development, from day 17 to 18 of gestation in the fetus to the first few days after birth. This antigen was detected in 11 of 12 human tumor intestinal cell lines. By immunofluorescent staining, it was found present only in subsets of the cultured tumor cells appearing as colonies of variable size. In contrast, it could not be detected in a large panel of normal rat and human cell lines. Expression of the YBB 3/10 antigen could be induced in the only negative colon tumor cell line (DLD-1 cells) by treatment with N,N-dimethylformamide (CAS: 68-12-2). These results suggested that the YBB 3/10 antigen represented a marker for the differentiated state of human colon tumor cells and that it would be useful to investigate the relationship between the state of cell differentiation and the pattern of development of small and large intestine during fetal and postnatal life.

UI	MeSH Term	Description	Entries
D007421	Intestine, Small	The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.	Small Intestine,Intestines, Small,Small Intestines
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003110	Colonic Neoplasms	Tumors or cancer of the COLON.	Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D004126	Dimethylformamide	A formamide in which the amino hydrogens are replaced by methyl groups.	N,N-Dimethylformamide,N,N Dimethylformamide
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000951	Antigens, Neoplasm	Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.	Neoplasm Antigens,Tumor Antigen,Tumor Antigens,Antigen, Tumor,Antigens, Tumor