Biomaterial Database

Prostaglandin E2 synthesis by human primary and metastatic bone tumors in culture. 1985

M C Gebhardt, and L Lippiello, and F R Bringhurst, and H J Mankin

Prostaglandin E2 (PGE2) is known to stimulate osteolysis in vitro and has been implicated in mediating bone resorption in several animal and human tumors. Little attention has yet to be directed toward local humoral control (including PGE2) of bone resorption in primary and metastatic bone tumors. For investigation of whether histologically identified areas of osteolytic or osteoblastic bone tumors differentially secrete PGE2 under in vitro conditions, culture media from explants of central and peripheral areas of tissue were sterilely collected from 13 surgical specimens of primary and metastatic bone tumors and assayed for (PGE2) radioimmunoassay. The results indicate a marked heterogeneity in the concentration of immunoreactive (I-PGE2) synthesis by tumors of different as well as similar cell type. PGE2 production was time-dependent in culture, and at 72 hours substantial increases were apparent compared to cultures of non-neoplastic fascia controls. Significantly higher levels of I-PGE2 were found in cultures derived from "bone-destructive" tumors. No difference in I-PGE2 synthesis was found between explants of peripheral versus central tissue of the same tumors. PGE2 is synthesized in culture by bone tumors characterized as destructive of bone at higher levels than "bone-forming" tumors, and this synthesis is inhibited by indomethacin.

UI	MeSH Term	Description	Entries
D009362	Neoplasm Metastasis	The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.	Metastase,Metastasis,Metastases, Neoplasm,Metastasis, Neoplasm,Neoplasm Metastases,Metastases
D010017	Osteoma, Osteoid	A benign osteoblastic tumor with central vascularized nidus surrounded by normal reactive bone. It occurs especially in second decade of life most commonly in the femoral neck but can occur in any bone and any site within a bone.	Osteoid Osteoma,Osteoid Osteomas,Osteomas, Osteoid
D011458	Prostaglandins E	(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.	PGE
D011459	Prostaglandins E, Synthetic	Analogs or derivatives of prostaglandins E that do not occur naturally in the body. They do not include the product of the chemical synthesis of hormonal PGE.	PGE Synthetic,Prostaglandin E Analogs,Prostaglandin E Analogues,Synthetic Prostaglandins E,Analogs, Prostaglandin E,Analogues, Prostaglandin E,Synthetic, PGE
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D001859	Bone Neoplasms	Tumors or cancer located in bone tissue or specific BONES.	Bone Cancer,Cancer of Bone,Cancer of the Bone,Neoplasms, Bone,Bone Neoplasm,Neoplasm, Bone
D001862	Bone Resorption	Bone loss due to osteoclastic activity.	Bone Loss, Osteoclastic,Osteoclastic Bone Loss,Bone Losses, Osteoclastic,Bone Resorptions,Loss, Osteoclastic Bone,Losses, Osteoclastic Bone,Osteoclastic Bone Losses,Resorption, Bone,Resorptions, Bone
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005870	Giant Cell Tumors	Tumors of bone tissue or synovial or other soft tissue characterized by the presence of giant cells. The most common are giant cell tumor of tendon sheath and GIANT CELL TUMOR OF BONE.	Cell Tumor, Giant,Cell Tumors, Giant,Giant Cell Tumor,Tumor, Giant Cell,Tumors, Giant Cell