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A method was developed for the in vitro induction by 12-O-tetradecanoylphorbol-13-acetate (TPA) of ornithine decarboxylase (ODC) activity in human skin punch biopsy samples. Addition of TPA to 1 ml serum-free minimum essential medium containing a single 3-mm human skin punch biopsy sample obtained from a surgical specimen resulted in an induction of ODC activity with a peak activity at 5 hours after TPA addition. In vitro induction of human epidermal ODC activity was dependent on the TPA concentration in the medium; about a twofold increase in ODC activity was observed 6 hours after the addition of 0.1 microM TPA, and about a fivefold increase in ODC activity was observed with 1 microM TPA. TPA also caused about a fivefold to sixfold increase in ODC activity in 3-mm skin punch biopsy samples from healthy volunteers. Human skin punch biopsy samples remained responsive to TPA induction of ODC activity even when stored in serum-free medium at 4 degrees C for 24 hours. A similar degree of induction of ODC activity by TPA was observed whether whole unfractionated human epidermis or a soluble epidermal extract was used for ODC assays. Increased ODC activity was the result of the increase in enzymatically active ODC protein, quantitated by a [3H]difluoromethylornithine-binding assay. Thus human skin, like mouse skin, is responsive to TPA for ODC induction.
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
September 1980,
Proceedings of the National Academy of Sciences of the United States of America,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
January 1986,
Cancer research,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
December 1980,
Biochemical and biophysical research communications,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
June 1986,
Archives of biochemistry and biophysics,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
July 1994,
Molecular carcinogenesis,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
July 1981,
Biochemical and biophysical research communications,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
February 1989,
Carcinogenesis,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
February 1981,
Experientia,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
January 1988,
Advances in experimental medicine and biology,
A K Verma, and
C L Loprinzi, and
R K Boutwell, and
P P Carbone
December 1983,
Cancer research,
