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Mouse myeloid leukemic M1 cells are induced to differentiate by various differentiation inducers. Activity for inhibition of induction of differentiation of M1 cells (I-activity) has been found in conditioned medium of variant M1 cell clones resistant to differentiation inducers, and this I-activity has been shown to be closely associated with resistance of the cells to differentiation inducers. In this work, the I-activity in the conditioned medium of the resistant M1 cells was shown to bind to Carboxymethyl-Sepharose CL-6B and to be eluted with 0.27-0.4 M NaCl. The profile of gel filtration of I-activity from Sephadex G-200 indicated considerable heterogeneity in molecules with I-activity; the apparent molecular range of the main I-activity was 60,000-80,000. On chromatofocusing, the I-activity was eluted with Polybuffer 96-acetic acid at pH 8.8-9.0. The I-activity was inactivated by treatment with trypsin or by heating at 75 degrees C for 30 min. Therefore, the main I-activity seemed to be due to a basic protein(s).
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
August 1977,
Gan,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
February 1983,
Cell differentiation,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
April 1985,
Biochemistry international,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
April 1977,
Gan,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
September 1979,
Biochemical and biophysical research communications,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
October 1992,
Leukemia research,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
February 1991,
Japanese journal of cancer research : Gann,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
February 1980,
Gan,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
December 1979,
Cancer research,
J Okabe-Kado, and
M Hayashi, and
Y Honma, and
M Hozumi
October 1979,
Biochimica et biophysica acta,
