-transparent.png){kind=logo}
A single polypeptide of leucyl-tRNA synthetase (LRS) has been purified from budding bakers' yeast by a modification of the procedure published earlier. On denaturing polyacrylamide gel electrophoresis LRS was one band corresponding to molecular weight of 120,000 +/- 5,000 daltons. Variable amounts of LRS with a similar molecular weight but which dissociated into equal subunits of 58,000 were also isolated. The affinities (KM) for substrates for this form of the enzyme were similar to those previously reported for the dimeric form of the enzyme.
Related Publications
C S Lin, and
R Irwin, and
J G Chirikjian
February 1973,
The Journal of biological chemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
January 1973,
Biochimie,
C S Lin, and
R Irwin, and
J G Chirikjian
December 1971,
European journal of biochemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
October 2015,
The Journal of biological chemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
May 1971,
Proceedings of the National Academy of Sciences of the United States of America,
C S Lin, and
R Irwin, and
J G Chirikjian
February 1977,
The Journal of biological chemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
May 1971,
European journal of biochemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
April 1979,
Molecular and cellular biochemistry,
C S Lin, and
R Irwin, and
J G Chirikjian
September 1967,
Biochemical and biophysical research communications,
C S Lin, and
R Irwin, and
J G Chirikjian
February 1987,
Proceedings of the National Academy of Sciences of the United States of America,
