BIOMAT Database Logo BIOMAT Database Logo

DNA polymerase delta governs parental histone transfer to DNA replication lagging strand. 2024

Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Chromatin replication is intricately intertwined with the recycling of parental histones to the newly duplicated DNA strands for faithful genetic and epigenetic inheritance. The transfer of parental histones occurs through two distinct pathways: leading strand deposition, mediated by the DNA polymerase ε subunits Dpb3/Dpb4, and lagging strand deposition, facilitated by the MCM helicase subunit Mcm2. However, the mechanism of the facilitation of Mcm2 transferring parental histones to the lagging strand while moving along the leading strand remains unclear. Here, we show that the deletion of Pol32, a nonessential subunit of major lagging-strand DNA polymerase δ, results in a predominant transfer of parental histone H3-H4 to the leading strand during replication. Biochemical analyses further demonstrate that Pol32 can bind histone H3-H4 both in vivo and in vitro. The interaction of Pol32 with parental histone H3-H4 is disrupted through the mutation of the histone H3-H4 binding domain within Mcm2. Our findings identify the DNA polymerase δ subunit Pol32 as a critical histone chaperone downstream of Mcm2, mediating the transfer of parental histones to the lagging strand during DNA replication.

UI MeSH Term Description Entries
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D004258 DNA Polymerase III A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms but may be present in higher organisms. Use also for a more complex form of DNA polymerase III designated as DNA polymerase III* or pol III* which is 15 times more active biologically than DNA polymerase I in the synthesis of DNA. This polymerase has both 3'-5' and 5'-3' exonuclease activities, is inhibited by sulfhydryl reagents, and has the same template-primer dependence as pol II. DNA Polymerase delta,DNA-Dependent DNA Polymerase III,DNA Pol III,DNA Dependent DNA Polymerase III,Polymerase III, DNA,Polymerase delta, DNA
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D006657 Histones Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. Histone,Histone H1,Histone H1(s),Histone H2a,Histone H2b,Histone H3,Histone H3.3,Histone H4,Histone H5,Histone H7
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D029701 Saccharomyces cerevisiae Proteins Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. Baker's Yeast Proteins,S cerevisiae Proteins
D064111 Minichromosome Maintenance Complex Component 2 A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It contains a NUCLEAR LOCALIZATION SIGNAL which may provide targeting of the protein complex and an extended N-terminus which is rich in SERINE residues. DNA Replication Licensing Factor MCM2,Minichromosome Maintenance Protein 2 Homolog,Nuclear Protein BM28,BM28, Nuclear Protein,Protein BM28, Nuclear

Related Publications

Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Idling by DNA polymerase delta maintains a ligatable nick during lagging-strand DNA replication.
November 2004, Genes & development,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
High-accuracy lagging-strand DNA replication mediated by DNA polymerase dissociation.
April 2018, Proceedings of the National Academy of Sciences of the United States of America,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
The PCNA-Pol δ complex couples lagging strand DNA synthesis to parental histone transfer for epigenetic inheritance.
June 2024, Science advances,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
An explanation for lagging strand replication: polymerase hopping among DNA sliding clamps.
September 1994, Cell,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Reconstitution of eukaryotic lagging strand DNA replication.
July 2010, Methods (San Diego, Calif.),
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. III. A polymerase-primase interaction governs primer size.
February 1992, The Journal of biological chemistry,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
The control mechanism for lagging strand polymerase recycling during bacteriophage T4 DNA replication.
January 2006, Molecular cell,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Differential correction of lagging-strand replication errors made by DNA polymerases {alpha} and {delta}.
December 2010, Proceedings of the National Academy of Sciences of the United States of America,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Mechanism of Lagging-Strand DNA Replication in Eukaryotes.
January 2017, Advances in experimental medicine and biology,
Congcong Tian, and Qin Zhang, and Jing Jia, and Jiaqi Zhou, and Ziwei Zhang, and Srinivasu Karri, and Jiuhang Jiang, and Quinn Dickinson, and Yuan Yao, and Xiaorong Tang, and Yuxin Huang, and Ting Guo, and Ziwei He, and Zheng Liu, and Yuan Gao, and Xinran Yang, and Yuchun Wu, and Kui Ming Chan, and Daoqin Zhang, and Junhong Han, and Chuanhe Yu, and Haiyun Gan
Enzymatic completion of mammalian lagging-strand DNA replication.
October 1994, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!