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We describe a competitive enzyme-linked immunoassay for Factor VIII antigen. Binding of anti-factor VIII to solid-phase Factor VIII antigen is competitively inhibited by the free factor VIII antigen that is to be measured. The amount of anti-Factor VIII bound to solid-phase VIII is measured by applying in sequence a heterologous bridging antibody and a soluble antibody/enzyme immune complex. The soluble complex used was rabbit antiperoxidase/horseradish peroxidase. Peroxidase activity is inversely proportional to the Factor VIII antigen concentration in the original test plasma and is measured spectrophotometrically. The assay can be performed in as little as 4 h with only a microtiter plate, antisera, antigen, and a spectrophotometer. It is sensitive to 0.05 units of Factor VIII antigen per milliliter, and reproducibility, linearity, and normal range are similar to those reported for other techniques.
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
January 1984,
Folia haematologica (Leipzig, Germany : 1928),
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
June 1982,
Clinical chemistry,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
October 1987,
Clinical chemistry,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
March 1985,
Journal of clinical pathology,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
April 1976,
British medical journal,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
October 1982,
Medical laboratory sciences,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
July 1980,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
January 1982,
Acta biologica et medica Germanica,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
February 1989,
Clinica chimica acta; international journal of clinical chemistry,
L D Yorde, and
C V Hussey, and
D E Yorde, and
E A Sasse
January 1986,
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao,
