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Peripheral blood mononuclear cells from eight patients with the rare T-cell form of chronic lymphocytic leukemia were isolated and cultured with Interleukin-2 (IL-2). In all but one case, cultured T-cells (CTC) were established. Various culture conditions were tested for their effectiveness; feeder layers proved valuable for expanding the cultures to large volumes. The CTC remained IL-2 dependent. Analysis of surface determinants on these CTC showed a polyclonal proliferation of T-cells. The distribution of subset markers in the patients' CTC population had completely changed in comparison to the "fresh" peripheral blood cell population but was similar to CTC initiated from healthy donors. Our data suggest that the patients' few contaminating normal T-lymphocytes expanded in culture, while the malignant cells were unresponsive to IL-2. This conclusion is supported by growth characteristics and morphology of the CTC.
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
February 2014,
The American journal of surgical pathology,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
June 1986,
Australian and New Zealand journal of medicine,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
January 1993,
Hematological oncology,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
July 2006,
Leukemia & lymphoma,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
April 1998,
Blood,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
June 2018,
Journal of translational medicine,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
June 1985,
Blood,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
January 1983,
Haematologica,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
October 1981,
Tumori,
M Gramatzki, and
F Pandolfi, and
J Maples, and
G De Rossi, and
G Semenzato, and
I Quinti, and
G Bonnard, and
D M Strong
September 1984,
Orvosi hetilap,
