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Fifteen independently isolated complementary DNA clones that contain T-cell receptor (TCR) V beta genes were sequenced and found to represent 11 different V beta genes. When compared with known sequences, 14 different V beta genes could be defined from a total of 25 complementary DNA's; 11 clones therefore involved repeated usage of previously identified V beta's. Based on these data, we calculate a maximum likelihood estimate of the number of expressed germline V beta genes to be 18 with an upper 95 percent confidence bound of 30 genes. Southern blot analysis has shown that most of these genes belong to single element subfamilies which show very limited interstrain polymorphism. The TCR beta-chain diversity appears to be generated from a limited V beta gene pool primarily by extensive variability at the variable-diversity-joining (V-D-J) junctional site, with no evidence for the involvement of somatic hypermutation.
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
September 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
February 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
September 1986,
The Journal of experimental medicine,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
August 1986,
Science (New York, N.Y.),
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
January 1984,
Nature,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
December 1989,
The Journal of experimental medicine,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
January 1987,
Immunogenetics,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
January 1990,
Experimental and clinical immunogenetics,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
January 1986,
Immunogenetics,
M A Behlke, and
D G Spinella, and
H S Chou, and
W Sha, and
D L Hartl, and
D Y Loh
April 1987,
Proceedings of the National Academy of Sciences of the United States of America,
