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A factor, termed neutrophil alkaline phosphatase-inducing factor (NAP-IF), that has the capacity to increase the NAP activity of granulocytes was characterized by using two samples: cystic fluid (CF) and conditioned medium of a tumor cell line (T3M5). The molecular weight of NAP-IF was shown to be between 13,000 and 45,000, and its isoelectric point was between 5.5 and 6.2. It was sensitive to heat and proteolytic enzymes, but was resistant to DNase and RNase, suggesting that NAP-IF is an acidic protein or glycoprotein. These characteristics of NAP-IF seem to be similar to those of granulocyte-macrophage colony-stimulating factor (GM-CSF) that is also present in the CF. NAP-IF rich fractions obtained by isoelectric focusing from CF were also found to be rich in a subclass of GM-CSF: granulocyte-CSF (G-CSF). Furthermore, a high correlation was noted between the activities of G-CSF and NAP-IF (gamma = 0.798, P less than 0.005). These results suggest that the two activities, i.e., G-CSF and NAP-IF, may be attributable to an identical macromolecule.
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
November 1976,
Journal of clinical pathology,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
December 2000,
Clinica chimica acta; international journal of clinical chemistry,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
May 1987,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
June 1966,
Rinsho byori. The Japanese journal of clinical pathology,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
July 1989,
Annals of internal medicine,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
March 1985,
Scandinavian journal of haematology,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
August 1985,
Scandinavian journal of haematology,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
December 2004,
Nihon rinsho. Japanese journal of clinical medicine,
N Sato, and
S Asano, and
M Mori, and
Y Ueyama, and
K Sugano, and
N Ohsawa, and
F Takaku
September 1999,
Nihon rinsho. Japanese journal of clinical medicine,
