-transparent.png){kind=logo}
To facilitate the direct study of progenitor cell biology, we have developed a simple and efficient procedure based upon negative selection by panning to purify large numbers of committed erythroid and myeloid progenitors from human fetal liver. The nonadherent, panned cells constitute a highly enriched population of progenitor cells, containing 30.4 +/- 13.1% erythrocyte burst forming units (BFU-E), 5.5 +/- 1.9% granulocyte-macrophage colony forming units (CFU-GM), and 1.4 +/- 0.7% granulocyte-erythroid-macrophage-megakaryocyte colony forming units (CFU-GEMM) as assayed in methylcellulose cultures. These cells are morphologically immature blasts with prominent Golgi. This preparative method recovers 60-100% of the committed progenitors detectable in unfractionated fetal liver and yields 2-30 X 10(6) progenitors from each fetal liver sample, and thus provides sufficient numbers of enriched progenitors to allow direct biochemical and immunologic manipulation. Using this technique, a purified recombinant protein previously thought to have only granulocyte-macrophage colony stimulating activity (GM-CSA) is shown to have both burst promoting activity and multipotential colony stimulating activity. Progenitor purification by panning thus appears to be a simple, efficient method that should facilitate the direct study of committed hematopoietic progenitors and their differentiation.
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
May 1988,
Proceedings of the National Academy of Sciences of the United States of America,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
January 1991,
Advances in internal medicine,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
May 2002,
Current opinion in hematology,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
January 1992,
Advances in pharmacology (San Diego, Calif.),
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
March 1987,
Proceedings of the National Academy of Sciences of the United States of America,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
March 1985,
Proceedings of the National Academy of Sciences of the United States of America,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
January 2004,
Experimental hematology,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
October 1990,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
January 1992,
Biotherapy (Dordrecht, Netherlands),
S G Emerson, and
C A Sieff, and
E A Wang, and
G G Wong, and
S C Clark, and
D G Nathan
May 1990,
Blood,
