-transparent.png){kind=logo}
A procedure to determine alpha-1-antitrypsin phenotypes (Pi types), including the six common PiM subtypes, is described. Phenotypes of the inhibitor, including the deficiency phenotype PiZ and the PiM subtypes, can be identified by isoelectric focusing in an immobilized pH gradient with a pH range of 4.2 to 5.0 or 4.0 to 5.0. This method gives clear separation of M, S, V, P and Z allele products. Up to 24 samples can be analyzed in a single overnight run using commercially available equipment and reagents.
Related Publications
K P Brooks, and
R M Iammarino
June 1979,
Jinrui idengaku zasshi. The Japanese journal of human genetics,
K P Brooks, and
R M Iammarino
January 1996,
Methods in enzymology,
K P Brooks, and
R M Iammarino
September 1988,
Electrophoresis,
K P Brooks, and
R M Iammarino
September 1978,
Scandinavian journal of clinical and laboratory investigation,
K P Brooks, and
R M Iammarino
October 1990,
Clinical chemistry,
K P Brooks, and
R M Iammarino
April 1992,
Electrophoresis,
K P Brooks, and
R M Iammarino
June 1998,
Electrophoresis,
K P Brooks, and
R M Iammarino
October 1997,
Journal of chromatography. B, Biomedical sciences and applications,
K P Brooks, and
R M Iammarino
December 1987,
Journal of biochemical and biophysical methods,
K P Brooks, and
R M Iammarino
April 1992,
Electrophoresis,
