Biomaterial Database

Large unilamellar lipid vesicles for use in therapeutic and diagnostic medicine. 1985

J W Freytag

Biomedical Products Department, E.I. du Pont de Nemours & Co., Inc., Glasgow Research Laboratory, Wilmington, Delaware 19898.

Large unilamellar lipid vesicles are prepared by a detergent dialysis procedure using beta-D-octylglucoside as the detergent. This procedure is nondenaturing and allows for the encapsulation of sensitive biological molecules. Vesicles prepared with the composition of 2 mol phosphatidylcholine and 1 mol cholesterol have a mean diameter of 200 nm and allow for the encapsulation of 150 molecules of alkaline phosphatase per vesicle without loss of activity. Stability studies show that less than 4 per cent of the original enzyme leaks from the vesicles over a 250 day period upon storage at 4 degrees C. A mechanistic model for vesicle formation is described to provide a clear understanding of the events occurring during the encapsulation stages.

UI	MeSH Term	Description	Entries
D008081	Liposomes	Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.	Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D003902	Detergents	Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.	Cleansing Agents,Detergent Pods,Laundry Detergent Pods,Laundry Pods,Syndet,Synthetic Detergent,Agent, Cleansing,Agents, Cleansing,Cleansing Agent,Detergent,Detergent Pod,Detergent Pod, Laundry,Detergent Pods, Laundry,Detergent, Synthetic,Detergents, Synthetic,Laundry Detergent Pod,Laundry Pod,Pod, Detergent,Pod, Laundry,Pod, Laundry Detergent,Pods, Detergent,Pods, Laundry,Pods, Laundry Detergent,Synthetic Detergents
D003956	Dialysis	A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Dialyses
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.