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The effects of triethyl lead chloride (TriEL) on the in vitro assembly and disassembly of microtubules (MTs) from porcine brain were studied by turbidometry at 350 nm and by electron microscopy. TriEL inhibited MT assembly at 50 microM concentration and caused an almost complete disassembly of preformed MTs. The drug depolymerized MTs more effectively than colchicine. Concentrations higher than 50 microM TriEL caused an aberrant assembly process. Fibers about 10 nm width were formed in addition to aggregates of amorphous material. In vivo TriEL also caused MT depolymerization in interphase and mitotic PtK-1 and Ehrlich ascites tumor (EAT) cells as monitored by indirect immuno-fluorescent staining of tubulin and electron microscopy. The extent of MT depolymerization was concentration- and time-dependent. Recovery occurred as early as 5 min after removal of the drug. The fluorescent actin pattern in PtK-1 cells typical of stress fibers and subcortical filaments seemed not to be altered by the presence of TriEL. The vimentin intermediate filament system was, however, rearranged as a juxtanuclear complex after TriEL treatment. Furthermore, TriEL effected the inhibition of cellular growth (100% inhibition at about 10(-5) M). Cytokinesis is prevented to a great extent, resulting in the formation of binucleate cells which can additionally possess some micronuclei.
H P Zimmermann, and
K H Doenges, and
G Röderer
June 1988,
The Science of the total environment,
H P Zimmermann, and
K H Doenges, and
G Röderer
January 1982,
Neurobehavioral toxicology and teratology,
H P Zimmermann, and
K H Doenges, and
G Röderer
January 1991,
Cell biochemistry and function,
H P Zimmermann, and
K H Doenges, and
G Röderer
November 1966,
La Medicina del lavoro,
H P Zimmermann, and
K H Doenges, and
G Röderer
June 1983,
Experimental cell research,
H P Zimmermann, and
K H Doenges, and
G Röderer
July 1983,
Toxicology and applied pharmacology,
H P Zimmermann, and
K H Doenges, and
G Röderer
January 1986,
Journal of toxicology and environmental health,
H P Zimmermann, and
K H Doenges, and
G Röderer
January 1983,
Neurotoxicology,
H P Zimmermann, and
K H Doenges, and
G Röderer
January 1987,
Neurotoxicology,
H P Zimmermann, and
K H Doenges, and
G Röderer
December 1969,
Clinica chimica acta; international journal of clinical chemistry,
