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The present study was undertaken to determine whether bovine adipocytes could bind and respond to insulin. Adipose tissue from young bulls showed little response to physiological and higher concentrations of insulin over a 3-h incubation. In contrast, insulin did stimulate glucose uptake during prolonged incubations and also increased the activity of pyruvate kinase; in each case, half-maximum stimulation was observed with concentrations of about 0.5 ng/ml. Specific binding of [125I] iodoinsulin to bovine adipocytes was observed; the binding capacity was greater than that of young female rats determined under the same conditions. Bovine adipocytes internalized insulin. Incubation of bovine adipocytes with insulin resulted in a concentration-dependent loss of insulin receptors from the adipocyte surface. In summary, bovine adipocytes possess the capacity to both bind and respond to insulin; these findings are discussed in relation to a recent report to the contrary.
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
December 1982,
Metabolism: clinical and experimental,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
December 1990,
Endocrinology,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
November 1982,
Horumon to rinsho. Clinical endocrinology,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
November 1983,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
November 1977,
The Journal of clinical investigation,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
January 1976,
Journal of lipid research,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
December 1982,
Cell biophysics,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
August 1988,
Molecular and cellular endocrinology,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
January 1987,
The Korean journal of internal medicine,
R G Vernon, and
E Finley, and
E Taylor, and
D J Flint
August 1979,
Diabetes,
