-transparent.png){kind=logo}
Among eighteen polypeptides synthesized in vitro from colicin El plasmid, one of the major products with a molecular weight of 59,000 was identified as colicin El by its immunological property, molecular size, and biological activity. In addition to this polypeptide, seven other polypeptides reacted with colicin El antiserum. Using EcoRI-cleaved colicin El DNA, a 56,000 dalton polypeptide of truncated colicin El was synthesized, but no polypeptide that reacted with colicin El antiserum was produced from SmaI-cleaved colicin El DNA. This fact indicates that the direction of transcription of colicin El structural gene is from SmaI site to EcoRI site in vitro. The immunity protein of a molecular weight of 14,300 and a component of relaxation proteins of a molecular weight of 64,000 were deduced by comparing the results of the gene expression in vitro of one-half (pAO100) and a quarter (pAO2) of colicin El plasmid. The directions of transcription-translation in the genes on the plasmid were discussed. The colicin El plasmid appears to have at least three transcriptional units.
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
November 1983,
Journal of bacteriology,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
July 1983,
Journal of bacteriology,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
May 1986,
Genetika,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
June 1986,
Genetika,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
November 1983,
Virology,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
January 1978,
Cell,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
January 1979,
Methods in enzymology,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
November 1976,
Nature,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
May 1984,
The Indian journal of medical research,
Y Ebina, and
F Kishi, and
T Nakazawa, and
A Nakazawa
March 1983,
Biochimica et biophysica acta,
