BIOMAT Database Logo BIOMAT Database Logo

Genotoxicity of Gamma Radiation Against Lymphocytes of Radiation Workers: The Cytokinesis-Block Micronucleus Assay. 2024

Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf

<b>Background and Objective:</b> Gamma irradiation induces genotoxicity, characterized by the formation of extra-nuclear bodies and left behind during the anaphase stage of cell division, often referred to as a micronucleus (MN). The present work aims to monitor exposure to ionizing radiation as a genotoxic agent in the lymphocytes of workers at radiation energy centers. <b>Materials and Methods:</b> The lymphocyte cytokinesis block micronucleus assay used and analyzed the correlation between the Nuclear Division Index (NDI), age, blood type and the number of micronuclei (MN). Blood samples were collected from 20 volunteers in heparin tubes, exposed to 2 Gy gamma rays and cultured <i>in vitro</i>. <b>Results:</b> A significant difference in the number of micronuclei between blood group A and blood groups A, B and AB. The Nuclear Division Index (NDI) value for lymphocytes of radiation energy center workers after gamma radiation was significant (1.74±0.1) but still within the normal range. Neither MN frequency nor NDI values correlated with age, but MN frequency showed a correlation with blood type. <b>Conclusion:</b> The gamma irradiation did not induce a cytostatic effect but proved genotoxic to the lymphocytes of radiation energy center workers. Notably, blood type A demonstrated higher sensitivity to gamma radiation.

UI MeSH Term Description Entries
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D005260 Female Females
D005720 Gamma Rays Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source. Gamma Wave,Gamma Radiation,Nuclear X-Rays,Radiation, Gamma,X-Rays, Nuclear,Gamma Radiations,Gamma Ray,Gamma Waves,Nuclear X Rays,Nuclear X-Ray,Ray, Gamma,Wave, Gamma,Waves, Gamma,X Rays, Nuclear,X-Ray, Nuclear
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D015162 Micronucleus Tests Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION. Micronucleus Assays,Assay, Micronucleus,Assays, Micronucleus,Micronucleus Assay,Micronucleus Test,Test, Micronucleus,Tests, Micronucleus
D016273 Occupational Exposure The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation. Exposure, Occupational,Exposures, Occupational,Occupational Exposures
D048629 Micronuclei, Chromosome-Defective Defective nuclei produced during the TELOPHASE of MITOSIS or MEIOSIS by lagging CHROMOSOMES or chromosome fragments derived from spontaneous or experimentally induced chromosomal structural changes. Chromosome-Defective Micronuclei,Genotoxicant-Induced Micronuclei,Micronuclei, Genotoxicant-Induced,Micronucleus, Chromosome-Defective,Chromosome Defective Micronuclei,Chromosome-Defective Micronucleus,Genotoxicant Induced Micronuclei,Genotoxicant-Induced Micronucleus,Micronuclei, Chromosome Defective,Micronuclei, Genotoxicant Induced,Micronucleus, Chromosome Defective,Micronucleus, Genotoxicant-Induced

Related Publications

Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Cytokinesis-block micronucleus cytome assay in lymphocytes.
January 2011, Methods in molecular biology (Clifton, N.J.),
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Cytokinesis block micronucleus assay in field plants for monitoring radiation-induced genotoxicity of the environment.
November 2014, Mutation research. Genetic toxicology and environmental mutagenesis,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Genotoxicity induced by arsenic compounds in peripheral human lymphocytes analysed by cytokinesis-block micronucleus assay.
July 2007, Mutagenesis,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
The assessment of genotoxicity of carbamazepine using cytokinesis-block (CB) micronucleus assay in cultured human blood lymphocytes.
January 2006, Drug and chemical toxicology,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Cytokinesis-block micronucleus cytome assay.
January 2007, Nature protocols,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Cytokinesis-Block Micronucleus Assay Using Human Lymphocytes as a Sensitive Tool for Cytotoxicity/Genotoxicity Evaluation of AgNPs.
June 2020, ACS omega,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Effects of the mycotoxin citrinin on micronucleus formation in a cytokinesis-block genotoxicity assay in cultured human lymphocytes.
January 2007, Journal of applied toxicology : JAT,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
In vitro evaluation of the genotoxicity of CeO2 nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX.
May 2018, Toxicology and industrial health,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
Laboratory intercomparison of the cytokinesis-block micronucleus assay.
August 2013, Radiation research,
Yulia Irnidayanti, and Marlina Azzahra, and Yanti Lusiyanti, and Devita Tetriana, and Darlina Yusuf
In vivo genotoxicity assessment of sertraline by using alkaline comet assay and the cytokinesis-block micronucleus assay.
November 2013, Basic & clinical pharmacology & toxicology,
Copied contents to your clipboard!