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We have cloned in Escherichia coli a DNA copy of mRNA coding for bovine preproparathyroid hormone. Double-stranded DNA was inserted into the Pst I site in plasmid pBR322 by using the poly(dG)-poly(dC) homopolymer extension technique to join the DNA molecules. Recombinant plasmids coding for preproparathyroid hormone were identified by the plasmid's ability to arrest specifically the translation of preproparathyroid hormone mRNA. The nucleotide sequence of the largest recombinant was determined by using both chemical and enzymatic techniques. The parathyroid insert contains 470 nucleotides--102 nucleotides from the 5' noncoding region of the mRNA, 345 nucleotides representing the entire coding region, and 23 nucleotides from the 3' noncoding region. The coding sequence clarifies the hormone's amino acid sequence, which has been disputed. Codon usage is discussed.
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
February 2002,
American journal of veterinary research,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
December 1981,
Proceedings of the National Academy of Sciences of the United States of America,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
December 1988,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
February 1989,
Molecular endocrinology (Baltimore, Md.),
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
October 1987,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
March 1979,
Proceedings of the National Academy of Sciences of the United States of America,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
March 1984,
The Journal of biological chemistry,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
August 1987,
Nucleic acids research,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
April 1982,
Nucleic acids research,
H M Kronenberg, and
B E McDevitt, and
J A Majzoub, and
J Nathans, and
P A Sharp, and
J T Potts, and
A Rich
October 1984,
Virology,
